Lab 11 new version - Lab11:Analysis of ptc gene sequence...

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Lab11:Analysis of ptc gene sequence and amino acid sequence of PTC receptor 1. Using NCBI database to compare with your ptc sequences: Blast (Today) This step can help you figure out if your DNA sequence can be classified as homozygous dominant(PAV/PAV) or recessive (AVI/AVI). However, we can not know your specific haplotype if you are heterozygous because a sequence text file could only show “N” when it contain two major nucleotide signal in one position. 2. Using Chromas lite to analyze chromatogram (Today) This step can help you figure out two haplotypes if you are heterozygous: PAV/AAV, PAV/AVI, AAV/AVI. 3. Using translation software and UniproKB for analyzing protein properties (Next week) 4.. Using PredictProtein software for estimating protein secondary and tertiary structure.(Next week)
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tas2r38 gene sequence and Single nucleotide pol ymorphism Single nucleotide polymorphism (SNP): a variation at a single position in a DNA sequence among individuals. If more than 1% of a population does not carry the same nucleotide at a specific position in the DNA sequence, then this variation can be classified as a SNP. If a SNP occurs within a gene, then the gene is described as having more than one allele. Therefore, SNPs may lead to variations in the amino acid sequence. Three positions on the Tas2r38 gene sequence vary and lead to different amino acid sequence on PTC receptor codon C CA or G CA G C T or G T T G TC or A TC 49 th amino acid --CCA: Proline ; GCA: Alanine 262 th amino acid --GCT: Alanine ; GTT: Valine 296 th amino acid --GTC: Valine ; ATC: Isoleucine
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Genetic concepts for PTC receptor study Haplotype : a group of genes within an organism that was inherited together from a single parent . A haplotype can describe a pair of genes inherited together from one parent on one chromosome, or it can describe all of the genes on a chromosome that were inherited together from a single parent. It can also refer to the inheritance of a cluster of single nucleotide polymorphisms (SNPs), which are variations at single positions in the DNA sequence among individuals.
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