PostPTCLabWorksheet - IB Biology 2 IHS Name Per Post-Lab...

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IB Biology 2 / IHS Name: _________________________________________________ Per. __________ Post-Lab: Using a SNP to Predict Bitter-Tasting Ability 1. Write the names of your teammates in the order (from left to right) they loaded their DNA in your gel. Consult the gel map if you don’t remember. __________________________ , _________________________ , __________________________ , _________________________ 2. Determine your PTC phenotype. First, place one strip of control taste paper in the center of your tongue for several seconds. Note the taste. Then, remove the control paper, and place one strip of PTC taste paper in the center of your tongue for several seconds. How do you and your teammates describe the taste of the PTC paper as compared to the control: strongly bitter, weakly bitter, or no taste other than paper? __________________________ , _________________________ , __________________________ , _________________________ 3. Determine your PTC genotype. Observe the photograph of the stained gel containing your PCR digest and those from your teammates. Orient the photograph with the wells at the top. Use the ideal gel photograph to help interpret the band(s) in each lane of your gel. a) Locate the marker DNA lanes on either side of the gel. Because our gels ran longer than the ideal gel, the bands of DNA appear more spread out than they are in the ideal gel. The marker was a plasmid called pBR322 that was digested with a restriction enzyme called Bst NI. Locate the bands corresponding to each restriction fragment: 1,857 bp, 1,058 bp, 929 bp, 383 bp and 121 bp. The 121-bp band may be very faint, even not visible.
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