7-DNA replication

7-DNA replication - DNA - Replication Chapter 12 Mechanism...

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DNA - Replication Chapter 12 Mechanism of replication 0. Possible models 0. conservative 1. semiconservative 2. dispersive fig.12.1, p.320 Mechanism of replication 1. Meselson and Stahl (1958) 3. replication is semiconservative fig.12.3, p.322 Requirements of replication
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2. DNA template 3. dNTPs (substrates) 4. enzymes Replication in Prokaryotes 5. Arthur Kornberg - 1955 4. E. coli replication 5. Nobel prize -1959 E.coli polymerases Polymerize 5' 3' 3' 5' exonuclease 5' 3' exonuclease DNA pol I yes yes yes DNA pol II yes yes no DNA pol III yes yes no Enzymes involved 0. Three DNA polymerases isolated from E.coli by Kornberg 0. 2 additional have been discovered 1. Common characteristics: 1. Catalyze phosodiester bond between 3'OH and 5'PO 4 2. Direction is always 5' to 3' 3. Can only add to existing 3' end 0. cannot initiate de novo Four Stages of Replication 0. Initiation 0. Unwinding (followed by priming) 0. Elongation 0. in both priming and elongation, bases are added according to the
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7-DNA replication - DNA - Replication Chapter 12 Mechanism...

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