atrazine - Eric Ma Section 1 Lab Partner: David Mach...

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Eric Ma Section 1 Lab Partner: David Mach Atrazine Degradation Report Abstract : The purpose of this experiment is to explore the utilization of xenobiotics, such as atrazine by bacteria. In the experiment, pseudomonas capable of atrazine degradation was inoculated onto a minimal medium with atrazine as the only carbon and nitrogen source. The isolates were then characterized by colony morphology, Gram reaction, and oxygen relationship using thioglycollate media. The isolates formed white, circular, and convex colonies, and were gram negative rods and strict aerobes, which matches pseudomonas. The isolates were grown overnight at 30 ºC in fresh media containing atrazine and 1 ml of culture was removed after 20 and 48 hours and frozen. The degradation of atrazine was analyzed using HPLC (reverse phase C-8 hydrophobic column) and mineralization assay. HPLC analysis indicated atrazine were degraded in the 20 hour sample and completely degraded in the 48 hour sample. HPLC from the 0 hour sample showed an atrazine peak at 1.78 minutes while the sample showed a peak at 1.7 minutes and the 48 hour sample showed a peak at 1.57 minutes. Mineralization assay indicated 9.53 x 10 -7 mmoles of 14 CO 2 was produced with the initial concentration of 14 C- atrazine being 6.84 x 10 -7 mmoles, which shows that 139 % of 14 C-atrazine was converted to 14 CO 2
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This note was uploaded on 05/06/2008 for the course MICROBIO 527 taught by Professor Paustian during the Spring '08 term at Wisconsin.

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atrazine - Eric Ma Section 1 Lab Partner: David Mach...

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