Brush_Table02_Lab10 - Erin Brush Partner Felicia Koerner...

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Erin Brush Partner: Felicia Koerner Lab 10: Diffraction Gratings I. Experiment Purpose and Design The purpose of this lab is threefold: 1) Find the wavelength of a laser light using a precut diffraction grating. 2.) Find the sarcomere length of rabbit muscle. 3.) Find the dimension of the hexagonal ommatidia of a butterfly eye. To do this, we shown a laser light through the diffraction grating, rabbit muscle slide, and butterfly eye slide accordingly and projected it onto a screen. We then measured the distances from the laser points to the center as well as the distance from the grating to the projection screen. These distances were then used to calculate the wavelength of the laser light. Using that wavelength, we were able to then diffract tissue samples and estimate what their actual dimension should be. II. Answers to Inline Questions • An expression for sin(θn), in terms of the observable distances x and y n (see Figure 4.1) was derived in the Pre-lab Quiz. The uncertainty in the sine of the angle δ(sin(θn)) is given. (See data for uncertainty of sine) • Be sure to report the measured wavelength with an uncertainty estimate δλ. (See results section) • Based on your measurements, what is the distance between Z-disks (sarcomere length)? • What is the estimated uncertainty? d = λ slope = 4.83 10 7 0.1725 = 2.77 10 6 m δd = ( slope δ λ ) 2 + ( λ δslope ) 2 = 9 10 9 m • From the diffraction pattern that you observe, what are the dimensions of the approximately hexagonal ommatidia?
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