Chapter 6 Summary

Kuby Immunology

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
Chapter 6 I. Antigen-Antibody Interaction: Principles and Applications 1. There are many noncovalent interaction between epitote of antigen and v-region of  antibody 2. Particularly the hypervariable regions B. Strength of Antigen-Antibody Interactions     1. Ag-Ab = Antigen-Antibody binding 2. Bonding includes hydrogen bonds, ionic bonds, hydrophobic interactions, and van  der Waals interactions a) A large number of such bonds are necessary for Ag-Ab strong bond b) These bonds require minimal separation – thus there must be high degree of  complementarity between Ag and Ab C. Antibody Affinity is a Quantitative Measure of Binding Strength     1. Affinity – the strength of the noncovalent interactions between a singles antigen- binding site on an Ab and a single epitote 2. Ka = affinity constant 3. Kd = dissociation constant 4. Surface Plasmon Resonance (SPR) – method to detwemin affinity contant 5. Equilibrium Dialysis 6. Scatchard Equation 7. Valency D. Antibody Avidity Incorporates Affinity of Multiple Binding Sites     1. Affinity at 1 binding site doesn’t always reflect true strength of Ab-Ag interaction 2. IgM has a high avidity
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
E. Cross Reactivity     1. Cross-Reactivity – when an antibody elicited by 1 antigen reacts w/ unrelated  antigen 2. ABO Blood-Group Antigens – glycolipids expressed in red blood cells F. Surface Plasmon Resonance (SPR)     1. allows measurements of affinity constants 2. 3. Resonant Angle G. SPR can be Used to Characterized the Epitope Specificities of Collections of Antibodies
Background image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 05/08/2008 for the course BIO 347 taught by Professor Sanders during the Spring '08 term at University of Texas.

Page1 / 4

Chapter 6 Summary - Chapter 6 I. Antigen-Antibody...

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online