genetics exam 1 problem sets

genetics exam 1 problem sets - Caitlin Favaloro Cbf229...

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Caitlin Favaloro Cbf229 Problem Set Chapter 9: C4: The building blocks of a nucleotide are a nitrogenous base, a sugar, and a phosphate. Nucleotides are linked together through a phosphodiester bond on the 5’ carbon of one sugar with the 3’ carbon of another sugar. C12: The nitrogenous bases mainly occupy the major and minor grooves of a DNA strand. The phosphate and sugar mainly occupy the backbone of a DNA strand. A DNA binding protein that does not recognize a specific nucleotide sequence would bind to the DNA backbone because nucleotide sequences mainly occupy a DNA’s grooves. C14: C15: 5’ AUCGGCAUUCGACCCCCCCCCCCCCCCCUCGAAUGCCGAU 3’ C20: Adenine= 18%, Thymine=18%, Cytosine=32%, Guanine=32% C29: Based on Chargaff’s rule, we can assume that the RNA is double stranded because of complimentary base pairing. However, we cannot know for sure because RNA can have both single stranded and double stranded parts. E2c: In the experiment on figure 9.3, although the nucleic acid affected the bacteria, it was still treated with DNase, RNase, and protease to remove any impurities within the nucleic acid. When the DNA was treated with DNase, the bacteria remained the same, proving that DNA was the substance that causes heritable changes. A1: a) Complimentary base pairing is the most exact in humans because our genome is the largest; therefore, experimental errors do not prevail as much in the final result. b) Although errors exist in the experiment, it is not considered a problem to apply Chargaff’s rule because the inconsistencies are a result of experimental error. Therefore, we must apply Chargaff’s rule because it is theoretically correct. c) It is important for Chargaff to analyze DNA from diverse species because evidence from just one could prove to be misleading. Analyzing a diverse group of species further supports his evidence.
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Chapter 11 Problem Sets: C9: The -35 and -10 and +1 sequences on the origin of replication are functionally important because transcription factors bind to the conserved sequence on the -35 nucleotide region; the TATA box located at the -10 region is where the DNA strands are separated; and the transcription start point begins at the +1 region of the origin.
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This note was uploaded on 05/09/2008 for the course BIO 325 taught by Professor Saxena during the Spring '08 term at University of Texas at Austin.

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genetics exam 1 problem sets - Caitlin Favaloro Cbf229...

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