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Unformatted text preview: About 225 mg of protein and 5815 units of LDH were found in the crude extract, giving a specific activity of 25.84. Following 40% ammonium sulfate fractionation, 185 mg of protein and 5531 units of LDH were measured, giving a specific activity of 29.90. Yield decreased by 5% and purification increased by 1.16 fold. No measurements need to be repeated for the next lab, since repeated measurements produced the same numbers with a small delta. Even though purification barely increased, these initial steps were important for removing components like fats and lipids, which would interfere with future assays....
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This note was uploaded on 05/10/2008 for the course BIO CHEM 153L taught by Professor Kim during the Spring '08 term at UCLA.
- Spring '08