midterm_1_2008_key

midterm_1_2008_key - YOUR NAME_ Student ID#_ MIDTERM 1...

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YOUR NAME__________________________ Student ID#_________________ 1 NPB 100 MIDTERM 1 SPRING, 2008 Your signature______________________ Please check this box if you do not want your exam returned in the bins in the hallway… Question Score Total 1 14 2 26 3 32 4 24 5 4 TOTAL 100
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YOUR NAME__________________________ Student ID#_________________ 2 Question 1. (14 points total) Here is a list of techniques for visualizing neurons. For each experiment below fill in the blank with one of these techniques. (Each technique may be used more than once or not at all!) Golgi stain Lucifer Yellow Immunohistochemistry Jellyfish fluorescent proteins Nissl stain 1a. (2 points) To look at the shape of a cell you have recorded from with a pipette electrode you would use Lucifer Yellow. 1b. (2 points) To determine which cells in the brain contain the enzyme used to manufacture acetylcholine you would use Immunohistochemistry. 1c. (2 points) To observe the shapes of many different neurons found in the rat olfactory bulb you would use Golgi stain. 1d. (2 points) To label different kinds of neurons in vivo in transgenic mice you would use Jellyfish fluorescent proteins . 1e. (2 points) To see whether blind mice have fewer neuronal cell bodies in layer 6 of visual cortex you would use Nissl stain. 1f. (2 points) To determine whether the disease multiple sclerosis affects the amount of ribosomes made by neurons you would use Nissl stain. 1g. (2 points) To determine the shape of the dendritic tree of perkinje cells in the cerebellum you would use Golgi stain. Question 2 (26 points total) You are studying a neuron in a newly discovered life form from outer space. The neuron is at room temperature. The threshold for firing action potentials is +10mV The neuron has three types of ion channels. Type 1 channels are permeable only to ion A Type 2 channels are permeable only to ion B. Type 3 channels are permeable only to ion C. Ions A and C are cations, ion B is an anion.
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midterm_1_2008_key - YOUR NAME_ Student ID#_ MIDTERM 1...

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