Lab #3 - Jonathan Price 203547464 Life Sciences 2 Kyle Kerm...

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Jonathan Price 203547464 Life Sciences 2 Kyle Kerm Dis 2F February 10, 2008 LS 2 Lab #3: Metabolism in Goldfish Intro: Metabolism is defined as the total chemical activity within an organism. There are two types of metabolic processes: anabolic and catabolic. The former involves the synthesis of important chemical building blocks, while the latter serves to break down molecules. The energy required to perform these reactions is through respiration, which requires oxygen. The amount of oxygen taken up by the organism correlates with its metabolic rate, which is the reason for which we are using oxygen concentration to measure rate of respiration. In this lab we are measuring oxygen consumption rate in goldfish, to see how fast their respiratory processes are. Goldfish are poikilothermic organisms whose body temperature changes when the environmental temperature changes, making the organism’s metabolic rate easier to manipulate. In this lab we will compare the rate of consumption of oxygen in a normal environment and then compare it to two of a multitude of variables; specifically in this lab we are comparing the unaltered goldfish to goldfish in nicotine-water and goldfish in water of a different temperature. The rationale for this is to see how different environmental factors will affect respiration in goldfish. Our hypothesis is that the nicotine-goldfish and the temperature-manipulated goldfish will have a metabolic rate significantly different from the unaltered goldfish (and therefore will produce a statistically significant oxygen concentration.) The null is that both of these altered types of goldfish will not have a metabolic rate that is statistically different from the unaltered goldfish. First, replace the deionized H2O in the Oxygen Chamber with 200mL of fish water, and let sit for 10 minutes. Then, weigh a cup full of 200mL of fish water, collect two
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fish from the tank, and weigh the fish+water to find the weight of the fish. We want to choose
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This note was uploaded on 03/05/2008 for the course LIFESCI 2 taught by Professor Pires during the Winter '08 term at UCLA.

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Lab #3 - Jonathan Price 203547464 Life Sciences 2 Kyle Kerm...

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