Lecture04-05_handout

Lecture04-05_handout - Johnson et al PNAS 2003 Steitz EMBO...

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What might you see if replication weren’t semiconservative?
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Cairns, J., J. Mol Biol. 1963
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Time Initiation Termination
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J.A. Huberman and A.D. Rigss, 1968, J. Mol. Biol.; and J.A. Huberman and A. Tsai, 1973, J. Mol. Biol.
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PYRIMIDINES PURINES N N R O O H H 3 C N N R NH 2 O R N N N N NH 2 N N R N N O H NH 2 T C A G ( J. Org. Chem. 1994, 59 , 7238; Synlett 1997, 341) ISOSTERES R F F H H 3 C R CH 3 F F D ISOSTERES R N N CH 3 R N N F H CH 3 Z H 1E+02 1E+03 1E+04 1E+05 1E+06 1E+07 1E+08 Efficiency Vmax/Km thymine difluorotoluene dTTP dGTP dCTP dATP ( Moran et al, J. Am. Chem. Soc. 1997, 119 , 2056-2057) Watson-Crick H-bonds are not required for efficient, specific replication - shape dominates
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Thumb Fingers Palm Kiefer et al. Nature 1998
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Unformatted text preview: Johnson et al. PNAS 2003 Steitz, EMBO 2006 E. coli : • DNA ~20Å diameter • Replisome ~3-400Å (polymerizing complementary base pairs) • Replication fork speed ~1000bp/s • Time to complete genome ~40 min (4MB) • Error rate <1 per 10 6 bases Scale up: • DNA ~ 1m (bicycle path) • Replisome ~ FedEx Truck (scanning, matching & painting complementary stripes) • Fork speed ~ 600 kph (375mph) • Error rate ~ 1 botched stripe pair per 170 km (106 miles) Nucleoprotein complex DnaA assembly Erzberger et al., NSMB 2006...
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This note was uploaded on 05/07/2008 for the course MCB 130 taught by Professor Schekman during the Spring '08 term at Berkeley.

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Lecture04-05_handout - Johnson et al PNAS 2003 Steitz EMBO...

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