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Carbohydrate Questions - J Lozo Questions for Carbohydrate...

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J Lozo Questions for Carbohydrate Laboratory 1. The carbohydrates that shouldn’t act as a reducing sugar are trehalose, sucrose, and sorbitol. Trehalose and sucrose wouldn’t act a reducing sugars because neither one of them has a free keto or aldo group. Sucrose contains the a-(b-2fructosyl)-glucoside. Trehalose contains the a(a-1-glucosyl) glucoside. Sorbitol won’t act as a reducing sugar, because it is a sugar alcohol already. 2. The carbohydrates that shouldn’t react to the phenol H2SO4 Method are sorbitol (because it is a sugar alcohol), and glyceraldehyde.. The ones in my groups testing that didn’t react at all is sorbitol. My group also experienced very low numbers for glyceraldehydes and rhamnose (.068 and .021) respectively. Polysaccharides may not react to this method because when they are broken into components, those components may not be simple sugars. Our starch readings were low, .040 which supports this conclusion as breaking down starch, you break it down into the two primary components of amylopectin and amylase.
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