VDJ Recombination - their 3 end flank D on the 5& 3 ends and flank J segments on their 5 end 12/23 Rule Only gene segments flanked by RSSs with

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VDJ Recombination V(D)J recombination is a mechanism of genetic recombination that occurs in vertebrates, which randomly selects and assembles segments of genes encoding a diverse repertoire of T cell receptor and immunoglobulin molecules. Each mature B-Cell and T-Cell has a unique VDJ locus due to VDJ recombination of DNA during cell development Leader sequence gets proteolytically cleaved Rearranging gene segments (RSSs) The V(D)J segments that rearrange are flanked by conserved sequences called RSSs. RSS flank V segments on
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Unformatted text preview: their 3' end, flank D on the 5' & 3' ends, and flank J segments on their 5' end. 12/23 Rule Only gene segments flanked by RSSs with dissimilar spacers can undergo VDJ recombination with one an other. This ensures that Vs don't pair with V's etc. Such an outcome will yield a non-functional protein. Ig promoters are actively transcribed when they're brought close to enhancer (this is only possible after rearrangement) Only 1/3 of rearrangements preserve the correct reading frame of the J segment...
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This note was uploaded on 05/06/2008 for the course MCB 150 taught by Professor Coscey during the Spring '08 term at University of California, Berkeley.

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