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Unformatted text preview: a week Cloning hybridomas from fusion Plate at limiting dilution (<1 cell/well) in 96 well plates. Allow clones to expand. Expand positive well and test for production of antibody of desired specificity in culture supernatant Benefits of Monoclonal vs. Polyclonal (1) Consistent (2) Limitless supply (3) Easier to test for cross-reactivity...
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This note was uploaded on 05/06/2008 for the course MCB 150 taught by Professor Coscey during the Spring '08 term at University of California, Berkeley.
- Spring '08