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Unformatted text preview: S2, S3 14. Add 10mLs of vanadanate/molybdate reagent to each of the 9 solutions 15. Press the nm button to select the wavelength 420nm. 16. Press the ATC button to select A in order to read absorbance 17. Fill a cuvette about 2/3 full with the blank, insert it in the cell holder, and close the door 18. Press the 0 ABS 100%t button to set absorbance level to zero. 19. Remove the blank, rinse with water , fill it 2/3 full with first standard and insert into cell holder. 20.Record absorbance on report sheet. Repeat with 4 other standards and 4 water samples 21. Graph solution vs concentration. 22.Calculate the average absorbance of the 3 water samples record and then use to determine the average concentration of phosphates in sample 23.Report value and the source of water to TA Hazards: dispose of everything in proper containers, wear gloves when handling vanadanate soltuion...
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- Spring '08
- Phosphate, Pi- Pump, cell holder