Lab_Report_#8 - Metabolic Effects of Elevated Glucose On...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
Metabolic Effects of Elevated Glucose On Human Retinal Pigment Epithelia Cells Jaime Figlan Hatham Shawqi Department of Physiology At Michigan State University Tuesday, March 10 th , 2008
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Abstract The purpose of this experiment was to examine the effects of high levels of extracellular glucose on the amount of lactate within hRPE cells. It was hypothesized that high levels of glucose would increase the amount of lactate within the cell. Since high levels of lactate are considered to represent tissue hypoxia and acidosis, a direct relationship between glucose levels and lactate levels would support the idea that elevated levels of glucose are harmful to cell tissues. The 22mM glucose hRPE cell line contained a greater amount of lactate per microgram of cytoplasmic protein than the 5.5mM sample. This suggests that the increased levels of extracellular glucose produced an increased amount of lactate. Since higher levels of lactate within the cell are an indicator of hypoxia and acidosis, chronically high levels of extracellular glucose can be harmful to the cells of human retinal pigment epithelial cells.
Background image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 05/16/2008 for the course PSL 475 taught by Professor Hootman during the Spring '08 term at Michigan State University.

Page1 / 4

Lab_Report_#8 - Metabolic Effects of Elevated Glucose On...

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online