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Lab_Report_#8 - Metabolic Effects of Elevated Glucose On...

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Metabolic Effects of Elevated Glucose On Human Retinal Pigment Epithelia Cells Jaime Figlan Hatham Shawqi Department of Physiology At Michigan State University Tuesday, March 10 th , 2008
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Abstract The purpose of this experiment was to examine the effects of high levels of extracellular glucose on the amount of lactate within hRPE cells. It was hypothesized that high levels of glucose would increase the amount of lactate within the cell. Since high levels of lactate are considered to represent tissue hypoxia and acidosis, a direct relationship between glucose levels and lactate levels would support the idea that elevated levels of glucose are harmful to cell tissues. The 22mM glucose hRPE cell line contained a greater amount of lactate per microgram of cytoplasmic protein than the 5.5mM sample. This suggests that the increased levels of extracellular glucose produced an increased amount of lactate. Since higher levels of lactate within the cell are an indicator of hypoxia and acidosis, chronically high levels of extracellular glucose can be harmful to the cells of human retinal pigment epithelial cells.
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