Lab_Report_#5 - Chemical Effects on Glucose Absorption In...

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Chemical Effects on Glucose Absorption In Intestinal Epithelia Jaime Figlan Hatham Shawqi Department of Physiology At Michigan State University Tuesday, February 19 th , 2008
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Abstract This purpose of this experiment was to determine whether or not sodium plays a role in the absorption of glucose across epithelial cells by inhibiting two sodium transporters using phlorozin and ouabain chemicals. Phlorozin is known to disrupt the SGLT-1 sodium transporter while ouabain inhibits sodium, potassium-activated ATPase activity. A higher concentration of glucose in the mucosal fluid than in the serosal fluid would suggest that sodium plays a role in the transport of glucose across the epithelial cells. The data shows that when intestines are incubated in solutions of both ouabain and phlorozin, a serosal to mucosal glucose concentration ratio of less than 1 is achieved. Intestines incubated in solutions with only Ringer’s solution had a serosal to mucosal glucose concentration ratio of 1.79. This supports our hypothesis that absorption of glucose is dependent on the ability of sodium to pass through the membranes of epithelial cells.
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Introduction The absorption and regulation of sodium in the cell is essential in order to maintain functional transportation in and out of the cell. The ability of the cell to maintain an electrochemical gradient using sodium is necessary for the transport of glucose out of the cell. In this experiment we will observe the activity of SGLT-1, a
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Lab_Report_#5 - Chemical Effects on Glucose Absorption In...

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