Lab_Report_#6 - Results A standard curve was successfully...

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Results A standard curve was successfully created for the concentrations of p-nitroaniline. As the concentration of p-nitroaniline increased from 0 micromolar to 100 micromolar, the absorbance also increased in a linear fashion (Figure 1). The lowest concentration of p-nitroaniline had the lowest absorbance, while the highest concentration of p- nitroaniline had the highest absorbance. The time course of L-BAPNA hydrolysis by trypsin was successfully recorded in Table II. Figure 2 shows that the enzymatic reaction begins to deviate from linearity between 5.5 and 6.0 minutes. The third part of the experiment examined what effect substrate concentration had on the reaction rate. As the substrate concentration increased, so did the reaction rate. The lowest substrate concentration, .15 mM, was consistent with the slowest reaction velocity, .005296 micromoles per minute. Similarly, the highest substrate concentration, .6 mM, produced the fastest reaction velocity at .020488 micromoles per minute (Table III).
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