Lab_Report_#6 - Results A standard curve was successfully...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Results A standard curve was successfully created for the concentrations of p-nitroaniline. As the concentration of p-nitroaniline increased from 0 micromolar to 100 micromolar, the absorbance also increased in a linear fashion (Figure 1). The lowest concentration of p-nitroaniline had the lowest absorbance, while the highest concentration of p- nitroaniline had the highest absorbance. The time course of L-BAPNA hydrolysis by trypsin was successfully recorded in Table II. Figure 2 shows that the enzymatic reaction begins to deviate from linearity between 5.5 and 6.0 minutes. The third part of the experiment examined what effect substrate concentration had on the reaction rate. As the substrate concentration increased, so did the reaction rate. The lowest substrate concentration, .15 mM, was consistent with the slowest reaction velocity, .005296 micromoles per minute. Similarly, the highest substrate concentration, .6 mM, produced the fastest reaction velocity at .020488 micromoles per minute (Table III).
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 05/16/2008 for the course PSL 475 taught by Professor Hootman during the Spring '08 term at Michigan State University.

Page1 / 2

Lab_Report_#6 - Results A standard curve was successfully...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online