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Unformatted text preview: BERG/STRYER VI STUDY GUIDE CHAPTER 5 1. HOMEWORK 1, 2, 3, 7. Look at the list under 5.1, page 135. We will briefly discuss each of these items except #4 . Restriction cleavage enzymes are made by bacteria as a defense mechanism against viruses. Each enzyme has a "palindromic" target sequence. The most useful enzymes make "lap joint" cuts. See EcoRI, BamH1, etc. in Fig 5.1. Using a restriction enzyme to cleave DNA leads to fragments of various random lengths. After separation by size in an agarose gel, Southern Blotting (Fig 5.3) transfers and "glues" the fragments onto nitrocellulose sheets. This is useful in looking for a particular gene, and also for comparing DNA from different individuals. Read about RFLP (box, page 137), used in forensic analysis by law enforcement workers Fig 5.9). The Sanger Dideoxy method of DNA sequencing is easy to understand if you know that you are growing random-length fragments of DNA. In a given test tube, all the growing fragments begin at exactly the same spot, and all end at the same nucleotide...
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This note was uploaded on 05/09/2008 for the course MOLBIO 315 taught by Professor Deis during the Spring '08 term at Rutgers.
- Spring '08