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BERG/STRYER VI STUDY GUIDE CHAPTER 16 GLYCOLYSIS 1. Homework 1, 2, 7, 11-13, 16, 20. Review main reaction types (Table 15.5 page 425) . Glycolysis is an anaerobic pathway, and very ancient. Parts of it are essentially universal. It takes place in the cytoplasm. You need to learn all intermediates with structures, all enzymes and all cofactors as shown in Fig. 16.2. Notice in that figure that 3 steps (all "kinase" reactions) are irreversible. Know which ones they are. In Table 16.1, know the approximate Δ G o ' for all reactions where the absolute value is 4 or greater. 2. Know the fates of pyruvate (Fig. 16.9) – anaerobic, to lactate; aerobic, to Acetyl CoA; yeast anaerobic, to CO 2 and ethanol. Understand how the anaerobic fates maintain redox balance. Two of the enzymes involved have the "Rossman fold" for binding NAD + just like G3PDH (Fig. 16.12). Know how galactose and fructose enter glycolysis, and understand the distinction between lactose intolerance and galactosemia. MECH: Know Lys Aldolase (not in book, but see Fig. 20.22) and G3PDH (440ff).
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