Unformatted text preview: of 2-8? 6. HPLC peaks generally should not have an asymmetry factor outside the range of 0.9-1.5. (a) Sketh the shape of a peak with an asymmetry factor of 1.8. (b) What might you do to correct the asymmetry? 7. (a) Nonpolar aromatic compounds were separated by HPLC on an odtadecyl (C18) bonded phase. The eluent was 65% methanol in water. How would the retention times be affected if 90% methanol were used instead? (b) Octanoic acid and 1-aminooctane were passed through the same HPLC column described in part (a), using an eluent of 20% methanol/80% buffer (pH 3.0). State which compound is expected to be eluted first and why....
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- Spring '08
- Chromatography, High performance liquid chromatography, high-performance liquid chromatography, Normal Phase Chromatography, eluent strength increases, eluent strength increase