Recombinant_DNA_SLIDES - Recombinant DNA Recombinant DNA is...

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Recombinant DNA Recombinant DNA is DNA that has been created artificially. DNA from two or more sources is incorporated into a single recombinant molecule. Making Recombinant DNA (rDNA): An Overview Treat DNA from both sources with the same restriction endonuclease (BamHI in this case). BamHI cuts the same site on both molecules 5' GGATCC 3' 3' CCTAGG 5' The ends of the cut have an overhanging piece of single- stranded DNA. These are called "sticky ends" because they are able to base pair with any DNA molecule containing the complementary sticky end. In this case, both DNA preparations have complementary sticky ends and thus can pair with each other when mixed. DNA ligase covalently links the two into a molecule of recombinant DNA.
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Plasmids Plasmids are molecules of DNA that are found in bacteria separate from the bacterial chromosome. They: 1. are small (a few thousand base pairs) 2. usually carry only one or a few genes 3. are circular 4. have a single origin of replication 5. Plasmids are replicated by the same machinery that replicates
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Recombinant_DNA_SLIDES - Recombinant DNA Recombinant DNA is...

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