PCR - Polymerase Chain Reaction: History and Impact of the...

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Polymerase Chain Reaction: By: Melissa Harintho, Jessica Spiewak,  Courtney McVey, Peggy Chang History and Impact of the PCR Revolution
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Polymerase Chain Reaction Introduction I. PCR Background Information II. Applications of PCR A. Forensic Science B. Human Genome Project C. Medical Diagnostic Tool D. Archaeology III.   Concluding Remarks
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 Before PCR was developed, DNA amplification    depended on cloning, which… -required a large quantity of DNA -dependent on other plasmids and vectors -very time consuming, laborious   Developed by Kary Mullis in the 1980’s at Cetus Corporation  Revolutionary because… -in vitro rather than in vivo -simplified procedure, more efficient -targeted specific DNA sequences -provided unlimited quantities of genetic material Polymerase Chain Reaction A Revolutionary Technique http://oregonstate.edu/dept/ncs/photos/mullis.jpg WOW! He’s  a genius!
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Polymerase Chain Reaction Procedure  http://concise.britannica.com/ebc/art-18071/The-three-step-process-of-the-polymerase-chain-reaction
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Polymerase Chain Reaction Problems Problems with PCR  INITIAL: Heating the DNA for  denaturing destroyed DNA polymerase  enzymes       SOLUTION: Taq polymerase  However, Taq polymerase has a high  copying error (approx. 40% will have  one incorrect nucleotide)       - Pyrococcus furiosus  ( Pfu      - Thermococcus Litoralis  (VENT)   http://waynesword.palomar.edu/images/hotspr5b.jpg http://plantphys.info/organismal/lechtml/archaea.html
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Polymerase Chain Reaction New Developments  RT PCR (reverse transcriptase)- transcribes RNA to cDNA to DNA      USE: very sensitive, good when RNA molecules are limited  Real Time PCR- emits fluorescent light through probe       USE: allows detection for progression of PCR http://kinich.cifn.unam.mx/~retligen/thermocycler.jpg
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Polymerase Chain Reaction Forensic Science • A run of repeated nucleotides is a 
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This note was uploaded on 05/18/2008 for the course BIOS 11161 taught by Professor Olsen during the Fall '08 term at Notre Dame.

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PCR - Polymerase Chain Reaction: History and Impact of the...

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