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Bio Lab 1 - Melissa Harintho Lab Section 1 Plantae South 1...

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Melissa Harintho Lab Section 1, Plantae South 1. a. (see attached) b. c. % error = [(experimental value- theoretical value)/ theoretical value] x 100% If we use the class average values as comparative “theoretical values,” we can calculate the percent error (a measure of discrepancy) between our bench’s data and the pooled data. % error: i. -30.2% ii. -32.3% iii. -16.1% iv. -14.8% v. -16.1% Our bench’s largest (numerical) deviation from the pooled data was 32.3%, and smallest deviation was 14.8%. Thus, our results demonstrate that while our results were relatively consistent (i.e. all of our percentage errors were slightly negative, or lower than the pooled data), they could have been more accurate (closer in value to the pooled data). 2. The Ve of hemoglobin was used for the calculation of Vo, because hemoglobin was the larger molecule; hemoglobin was too large to enter the pores of the gel bedding. 3. a. Rf values for TLC using solvent F: Bench Data Pooled Data i. 2 2.867 ii. 6 8.867 iii. 0.774 mL 0.922 mL iv. 1.775 mL 2.084 mL v. 0.774 mL 0.922 mL Dye Bench Data Pooled Data A 0 0.03 B 0.733 0.60 C 0.4 0.46 D 0.733 0.79
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b. Our values do not differ greatly from the lab average; the greatest deviation was for Dye B. This may have occurred if our plate was slightly tilted while it was in the beaker.
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c. Rf values for TLC using solvent G:
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