Bio Lab 3 - Melissa Harintho Lab Section 1, Plantae South...

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Melissa Harintho Lab Section 1, Plantae South 1. a. The overall charge on Protein A is negative. b. The overall charge on Protein B is also negative. c. Protein B has a greater magnitidue of charge. d. Protein A has a higher pertentage of basic amino acid residues. 2. If amine groups are ionized on protein, ionization lends a positive charge to the proteins. If carboxyl groups are ionized on proteins, ionization lends a negative charge to the proteins. 3. If I forgot to add glycerol to my sample buffer, the sample would not sink to the bottom of the sample wells. 4. Acetic acid, which fixes or precipitates, or immobilizes the proteins in the gel matrix after electrophoresis, should be used in the staining solution added to the sample mixture before loading the wells. 5. In electrophoresis, a protein will move towards the positive or negative electrode based on whether the pH of the chamber buffer is higher or lower than its pI. If the pH is equal to the pI, there will be no difference between the two values, and so the
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Bio Lab 3 - Melissa Harintho Lab Section 1, Plantae South...

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