Bio Lab 8 - Melissa Harintho Lab Section 1 Plantae South 1...

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Melissa Harintho Lab Section 1, Plantae South 1. AraC is the binding protein that binds to DNA at the binding site for RNA polymerase in the presence of arabinose (a sugar that changes the 3-D shape of araC). Arabinose is the inducer; in its presence, transformed bacteria will produce GFP, indicating that regulated transcription has been “switched on.” PBAD is the single promoter that allows for the transcription of 3 proteins (araB, araA, and araD) when initiated. RNA polymerase performs transcription of structural genes; in this case, genes B, A, and D. 2. E. coli is a bacteria that is single celled (allows for the insertion of the new gene(s) into just one cell as opposed to many cells of a multi cellular organism), reproduces very quickly (allows for experimental efficiency), and naturally produces enzymes needed to digest arabinose as a food source (demonstrates regulated expression naturally). 3. A “bacterial lawn” contains diffuse growth; because there are no selectable markers,
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Bio Lab 8 - Melissa Harintho Lab Section 1 Plantae South 1...

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