Activity%2023%20%20DNA%20PCR%20and%20Forensics-Key - Name...

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Name: BIOL1107 Activity #23 DNA, PCR, and Forensics 1) Polymerase Chain Reaction (PCR) mimics DNA replication in many ways. Five enzymes important for replication are listed below. Indicate if the function performed by each enzyme is needed for PCR and, if so, how that function is carried out. Replication PCR Helicase SSB Primase DNA Polymerase Ligase 2) Use the DNA molecule shown above, to answer the following questions: a) You use the DNA primer 5’ATAGTT 3’ to initiate replication of the DNA molecule above. i) Explain which strand (top or bottom) will be used as the template for new DNA synthesis and why. ii) Write out the sequence of the newly synthesized DNA strand starting from the 5’ end of the molecule. b) Write out the sequence of two 6 nucleotide long DNA primers that would allow you to amplify the entire DNA fragment .. 5' TACTCATTTAACTATGACGCTGTAACTTGT - 3' 3' ATGAGTAAATTGATACTGCGACATTGAACA - 5' High heat: breaks hydrogen bonds between nucleotides holding strands together Heat: also
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