Activity%2022%20DNA%20Replication-Key

Activity%2022%20DNA%20Replication-Key

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BIOL1107 Activity #22 DNA Replication 1) Most organic molecules, including the DNA molecule shown at the right, contain the nitrogen isotope N 14 . This DNA molecule is allowed to go through one round of DNA replication in the presence of adenine triphosphate nucleotides that contain the isotope N 15 . Write out the sequence(s) of the DNA molecule(s) produced as a result this replication. Mark each nucleotide in the resulting DNA molecules that will contain N 15 with an asterisk (*). 2) The figure at the right shows a partially drawn replication fork. a) Annotate this figure to show the proper location of each of the following: template DNA strand polarity leading strand daughter fragment(s) lagging strand daughter fragment(s) Single stranded binding proteins DNA polymerase Primase Ligase Helicase b) Explain why replication of the leading and lagging strands differ. 5’A ATTCCGG 3’ 3’ TTAAGGCC 5’ 5’AATTCCGG 3’ 3’TTAAGGCC 5’ ** ** 5’AATTCCGG 3’ 3’TTAAGGCC 5’
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