Lab Quiz 4 Questions

Lab Quiz 4 Questions - 1. What's the first fraction you...

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1. What’s the first fraction you obtain after centrifugation? a. nucleus b. What’s the next fraction? i. Chloroplast/mitochondria 2. What are “Matched Antibody Pairs”? a. Sandwich elisa – primary (capture), secondary (detection) have similar affinities for immunogenic proteins….used to determine antigen 3. What is malonate? What was the purpose of using malonate in our lab? a. Malonate is a competitive inhibitor of the SDH enzyme, mimics succinate b. We wanted to see it’s affect on the activity of SDH within the mitochondrial fraction 4. Why GAPDH? a. GAPDH – housekeeping gene. Because we had such small amounts of RNA we had to normalize it against an internal standard, therefore we utilized GAPDH, a housekeeping gene, for this normalization. 5. Definitions of Indirect/Direct ELISA. Which ELISA to determine patient antibody? a. Direct – uses antibodies to determine antigens b. Indirect – uses antigens to determine antibodies c. Indirect used to determine patient antibody titer. 6.
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This note was uploaded on 05/21/2008 for the course BIO 218 taught by Professor Castronuevo during the Winter '08 term at Drexel.

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Lab Quiz 4 Questions - 1. What's the first fraction you...

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