Ames Test Intro

Ames Test Intro - March 7, 2007 Biology 214 A modified Ames...

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March 7, 2007 Biology 214 A modified Ames test to examine chemicals that may increase the frequency of streptomycin resistance in a population of cells In this experiment, we preformed a modified Ames test with Escherichia coli to check for mutagenicity in peanut butter, Bath and Body Works Body Spray, ketchup, and soap. The Ames test assumes that any substance that is mutagenic may also turn out to be carcinogenic (Biology 214 Laboratory Manual). A normal Ames test uses a strain of Salmonella typhimurium that carries a mutant gene making it unable to synthesize histidine (Fratianni, et al., 2006 ). The regular test also includes a mixture of rat liver microsomal enzymes because many chemicals are not mutagenic or carcinogenic in themselves, but become mutagens and carcinogens as they are metabolized in the body (Fratianni, et al., 2006 ). Mutagenesis is the development of mutations in the genome (Campbell, 2005), and according to the Ames test theories, these mutations can lead to a cancerous condition in the cell (Biology 214 Laboratory Manual). The Ames test was preformed to see if our four substances are mutagens.
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This note was uploaded on 05/22/2008 for the course BIOL 214 taught by Professor Swanson during the Spring '07 term at Case Western.

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Ames Test Intro - March 7, 2007 Biology 214 A modified Ames...

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