Bio 214 re lab

Bio 214 re lab - Biology 214 Restriction Enzyme Results and...

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April 11, 2007 Biology 214 Restriction Enzyme Results and Discussion The agarose gel electrophoresis of lambda Hind III, Eco RI digested, Pst I digested, and Lambda DNA showed that the fragments varied in size (Figure 1). Lambda DNA has the largest fragments which is obvious because they migrated the shortest distance. Fragments that are the same size will move together through the gel and form bands (Biology 214 Laboratory Manual). From the known number of base pairs in Hind III, estimations can be made about the other samples (Table 1).The farther the band migrates the fewer base pairs there are. To more accurately estimate the unknown DNA band sizes, a standard curve based upon the band sizes of the Hind III bands can be made (Figure 2). Figure 1. Agarose gel electrophoresis photograph. The wells, from top to bottom are the marker, Lambda DNA, Pst I digested, Eco RI digested, and lambda Hind III.
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0 1 2 3 4 5 0 10 20 30 Distance Migrated (mm) Log base pairs Figure 2. The distance migrated in millimeters of Hind III in log base pairs. After the standard curve is made the size of the known fragments from the DNA standards can be used against the distance each DNA band moved through the gel, to
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This note was uploaded on 05/22/2008 for the course BIOL 214 taught by Professor Swanson during the Spring '07 term at Case Western.

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Bio 214 re lab - Biology 214 Restriction Enzyme Results and...

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