UnknownSProposal copy

UnknownSProposal copy - May 1, 2008 1:00pm/Thurs Title:...

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May 1, 2008 1:00pm/Thurs Title : Spectrophotometer Analysis Of an Unknown Solution Purpose : Using the principles of spectrophotometer, one must determine the concentration of the analyte S in the unknown sample given. In doing this, the extinction coefficient and the λ max for unknown S will be acquired as well. Plan of Procedure : 1. Determine the λ max of S using the pure analyte. To ensure that it is reasonably accurate, the maximum absorbance should be < 1.2. For an absorbance that is too high, the solution should be diluted. 2. To obtain the standard curve, determine the absorbance of various concentrations of the pure analyte S. Use to the value of λ max that was previously determined to measure the absorbance. Measure the absorbance of at least 6 different concentrations of S. The highest concentration of the standard should have an absorbance < 1.2, this would be best. The other five standards should have an absorbance of between 0.4 and 1.2. Then decide which concentrations of
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This note was uploaded on 05/23/2008 for the course CHEM 13 taught by Professor Suljak,steven during the Spring '08 term at Santa Clara.

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