Lecture 19 & 20

Lecture 19 & 20 - Lecture 19 20 Molecular Markers...

Info iconThis preview shows pages 1–7. Sign up to view the full content.

View Full Document Right Arrow Icon
Lecture 19 & 20: Molecular Markers & Their Use In Mapping Ke y wo rds Ke y Fig ure s S e q ue nc e  Ta g g e d S ite  (S TS ) Ta b le  20.1 Ha plo type   20.8 Po lym o rphis m 20.12 S a te llite  DNA (m ic ro ) 19.17 Fing e rprint ( pg  540-543 ) 19.19 Chapt. 20 Problems (Start on pg. 572) - S2, S4, C3, Chapt. 19 Problem - E17 (pg. 549)
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
How do we get a high density map in humans? (Meaning lots of markers for linkage analysis)
Background image of page 2
What do we need in human markers? 1. Must be in all humans 2. Show variation in the human population (be  polymorphic) Marker: RFLP Microsattelites Technology: Southern PCR The great thing is that these are “codominant” - you see both alleles Molecular markers (STSs) fit the bill: “VNTRs”
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Types of DNA Polymorphism s
Background image of page 4
Types of polymorphisms useful for mapping: 1. Single-base differences in sequence (~3X10 6 /human genome) 2. Satellite DNA sequences: micro/ mini satellite Microsatellite (100,000/human  genome) Minisatellite  (1000/genome) There are others, but we don’t cover them all.
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Detecting microsatellite polymorphism : Use PCR
Background image of page 6
Image of page 7
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 21

Lecture 19 & 20 - Lecture 19 20 Molecular Markers...

This preview shows document pages 1 - 7. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online