Sample Test Questions

Sample Test Questions - 12/2/2007 5:16:00 PM Sample Test...

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02/12/2007 17:16:00 Sample Test Questions What is RNAi?  What is the general mechanism?  What is the central dogma of  molecular biology?  How does RNAi contradict this idea?  What is the difference  between miRNA and siRNA? RNAi or RNA interference, or the inhibition of specific mRNA translation.  A  protein (DICER) recognizes dsRNA and cleaves it into 20-22 nt fragments.  These  fragments are utilized by the RISC (RNA Induced Silencing Complex) to seek out  mRNA transcripts that can anneal to this ~21nt sequence and target them for  destruction.  The central dogma is DNA   RNA   Protein and this mechanism shoes  that proteins can affect RNA levels directly and RNA can control DNA expression levels.  miRNA are small 20-22nt genomic sequences that are used for gene regulation.  siRNAs are 20-22nt fragments that are generated in response to finding a dsRNA  molecule and in turn used to knock down the corresponding protein levels If a scientist is studying vulva development in  C. elegans , how can he/she utilize  RNAi technology to determine if a protein ( vulvamaker ) is necessary for induction? A scientist could potentially introduce an dsRNA molecule against a particlular  protein involved in vulva formation.  If this RNAi molecule could potentially inhibit the  development of the vulva it would be possible to dedeuce that the protein is necessary 
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This note was uploaded on 05/26/2008 for the course MCDB 120 taught by Professor Johncarlsoncarolbascom-slackfrankslack during the Fall '07 term at Yale.

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Sample Test Questions - 12/2/2007 5:16:00 PM Sample Test...

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