biostudy session lecture

biostudy session lecture - 7:12:00 PM PCR Starts with a...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
25/10/2007 18:12:00 PCR: Starts with a double stranded molecule.  Heated in order to denature it leaves you with a single stranded DNA molecule. add oligonucletide primers. add polymerase You repeat the replication cycle over thirty times so that you have a lot of  DNA molecules to use. PCR: Cycles. 3 steps to each cycle o denature with heat to make two single strands o decrease heat to allow primers to bind. Anneal.  o make second strand. Have polymerase synthesize the strand of DNA.  Extend.  October 8 th  Lecture: Viruses, Bacteria, Gram Stain. Classes of Viruses Graham stain graham positive and graham negative. g negative has an extra outer membrane stain isn’t able to permeate all the way through. G+ bacteria are stained  because it is able tor each cell wall more easily without the extra outer  membrane Graham negative bacteria don't take up the stain. Graham positive has  crystalviolet. Crystal violet stains purple. G negative bacteria appear pink  Picture depicts inner and out membrane of bacteria and cytoplasm inside
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 05/26/2008 for the course MCDB 120 taught by Professor Johncarlsoncarolbascom-slackfrankslack during the Fall '07 term at Yale.

Page1 / 5

biostudy session lecture - 7:12:00 PM PCR Starts with a...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online