MCDB LAB FLOWCHART

MCDB LAB FLOWCHART - (1.5mL of N-Octyl alcohol in 150ml of Medium A 5mL of solution placed into Centrifuge Tube Homogenization Tube Shaken

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Drop of Tetrahymena + Drop of Methyl Green Pronin Solution Incubation for Ten Minutes Separate Tetrahymena from Growth Medium Two minute centrifugation at highest speed Put supernatant in clean beaker Suspension of cells Pellet is placed in 5mL of Medium A (.1M Sucrose 1.5 mM MgCl 2 ; 4% Gum Arabic and .01%spermindine HCl) Solution is centrifuged at highest speed for 2 minutes Supernatant is removed Pellet is suspended in 5ml Medium B*
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Unformatted text preview: *(1.5mL of N-Octyl alcohol in 150ml of Medium A) 5mL of solution placed into Centrifuge Tube Homogenization Tube Shaken Vigorously (breaking cells to release nuclei) Tube is Centrifuged Cloudy Supernatant and floating skin is removed Pellet Resuspend in 1.0mL of Medium A Combine two 1mL nuclear preparations Use Hemacytometer to count the number of nuclei/mL...
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This note was uploaded on 05/26/2008 for the course MCDB 121 taught by Professor Johncarlsonmariamoreno during the Fall '07 term at Yale.

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MCDB LAB FLOWCHART - (1.5mL of N-Octyl alcohol in 150ml of Medium A 5mL of solution placed into Centrifuge Tube Homogenization Tube Shaken

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