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Unformatted text preview: *(1.5mL of N-Octyl alcohol in 150ml of Medium A) 5mL of solution placed into Centrifuge Tube Homogenization Tube Shaken Vigorously (breaking cells to release nuclei) Tube is Centrifuged Cloudy Supernatant and floating skin is removed Pellet Resuspend in 1.0mL of Medium A Combine two 1mL nuclear preparations Use Hemacytometer to count the number of nuclei/mL...
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- Fall '07
- RNA, Centrifuge, centrifuged, highest speed, Tube Homogenization Tube, Methyl Green Pronin, clean beaker Suspension