This preview shows pages 1–2. Sign up to view the full content.
This preview has intentionally blurred sections. Sign up to view the full version.View Full Document
Unformatted text preview: *(1.5mL of N-Octyl alcohol in 150ml of Medium A) 5mL of solution placed into Centrifuge Tube Homogenization Tube Shaken Vigorously (breaking cells to release nuclei) Tube is Centrifuged Cloudy Supernatant and floating skin is removed Pellet Resuspend in 1.0mL of Medium A Combine two 1mL nuclear preparations Use Hemacytometer to count the number of nuclei/mL...
View Full Document
This note was uploaded on 05/26/2008 for the course MCDB 121 taught by Professor Johncarlsonmariamoreno during the Fall '07 term at Yale.
- Fall '07