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Problem_set_11_Key

# Problem_set_11_Key - = 0.5 V,n 0.1| rrrrM C Vo = 0.8 V"...

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* Huxford-Spring 2008 Chem 365 Biochemistry, Molecular & Cell Biology I Practice problem set 11 1) From VVP, Chapter 11 Problems 1,2,4,5, and 18. Chapter 12Problem 10 and 19 and the exercises that follow: For questions 2-5, consider the enzyme mechanism represented by the following chemical equation: k2 + E + P k-1 Which is NOT an accurate description of the steady state approximation? A) dlESl/dt = 0 BJ krlEllsl = k-'[ES] + ftrtEsl @ oplrot D) the rate of ES complex formation and break down are equivalent Which statement regarding K" is NOT true? A) It is defined as (fr_, + fu)lk, B) It is equal to [E][S]/ES] (Kr), if k.1>> lq Q) lt is the substrate concentration at 0.5 V."* @ tt has units of concentration/unit time (Molar sec-t) Which of the following is NOT equivalent to V*"* during steady state conditions? A % as [Sl approaches infinity p) KMtz c) ft,lE]r D) dlPl/dt, [S] >> Kna required for: A) Vo=0.1 V,"* {.023r,,r|u\ B) Vo = 0.5 V,n*

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Unformatted text preview: = 0.5 V,n* 0.1| rrrrM C) Vo = 0.8 V."* 0. t{ r". M D) Vo = 0.99 V,"* 20.6 ^ 14 E) How many times more ATP is needed to reach 0.99 V.u^ than 0.1 V.o E + s + E S 2) 3) 4) lx(o?s'f- ?rtorfr ls-l Vo : V'"*[s] ffiT 1.4'10-trn 2o,8r,rla. = 0, D23n^rt4 gql A)@ Vo 3 0, (V*'* o^l k^-- 0. zt ^h r 0.ozl'fn +0,t[s]=[s] [s]=ry 0.q 0.02J^14 0.1 Vnn + --.Vn** [s] 0,t1+ fsf Huxford-Spring 2008 7) For an enzyme-catalyzed reaction, the initial velocity was determined at two different concentrations of the substrate: l"Sl (mM) 1.0 4.0 Determine the values of K" and V,*. Vo = V** fs) K^-- |sf A - -g LSJ, : l,0rnM 2.0 ula /h; = @ fsl, = (.0 H/t^ 2,9 utAlr,^^ 2 g = (zr.-*tI+) Ku+( f. z Kr: 3.2 V^* V^ry, = lKr^t2 = (t-f,l, Zup+,*/ ^/ ^e I i - - n V"(mM/min) 2.0 2.8 L2) I ( 7vffit ,'-+,,roD ( ) {.tKr^ + Il.L= (kn +3 f K" , O,tz^nf_ + 2 : 0^;tL.o,*rnJ &^ + t,o MU) 'zKM+ 2,r^[T'/*^ = VA ku+ (,omhJ...
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