Exam 3 Answer Key

Exam 3 Answer Key - Exam 3 MIBO 2500 Spring '08 Dr. Walker...

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Exam 3 MIBO 2500 Spring ’08 Dr. Walker Name_______________________ There are 50 questions, each question worth 2 pts, totaling 100 points. Please be sure to double check that your answers from this test paper and those on the scantron are the same. Hand in the scantron and take home the test paper. 1.Which of the following must occur for homologous recombination? a. RNA is naturally taken up by the cell b. DNA fragments are ligated together by ligase c. Foreign DNA finds a similar region in the cell’s chromosome d. Host polymerases do not chew up foreign proteins acquired from the environment e. A transposon removes itself from the chromosome and inserts itself into a plasmid 2. Which of the following forms of gene transfer could involve homologous recombination? a. Conjugation with a F + plasmid b. Conjugation with Hfr c. Transformation by natural competence d. B and C e. A, B and C 3. If a donor Hfr cell mates with a recipient F - (minus) cell and only part of the plasmid and chromosome is transferred, what is the final status of the recipient cell? a. F + (plus) b. F - (minus) c. Hfr d. F e. P 4. What type of genetic information is obtained when a naturally competent cell undergoes transformation? a. Double stranded DNA b. A plasmid c. A chromosome with a plasmid inserted d. Single stranded DNA fragment e. Phage DNA 5. With generalized transduction __________ is transferred by phage to a new cell and the newly infected cell ______ produce new phage. a. Any random gene; can b. Any random gene; cannot c. A particular gene; can d. A particular gene; cannot e. A plasmid; can 6. Where can a transposon move by itself? a. From one enzyme to another b. From one cell to another c. From one site on a RNA strand to another d. From a plasmid to a chromosome e. From a phage DNA in one phage to DNA in another phage
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7. Promiscuous plasmids are often responsible for a. New bacterial infections via transformation b. New infectious viruses via transduction c. Acquired antibiotic resistance by a new genus and/or species via conjugation d. New microbial metabolic activities via recombinant DNA e. Antigen resistance by viruses and bacteria 8. If a nonsense mutation occurred in the gene encoding for the protein that make up the majority of the sex pilus in a F+ cell, which process(es) would be inhibited? a. Conjugation b. Transformation c. Transposons d. Filamentous phage infection e. A and D 9. Under what circumstances might a transposon be able to move to another cell? a. Transformation of a plasmid containing the transposon b. Generalized transduction of a segment that contains the transposon on the chromosome c. Conjugation of a plasmid containing the transposon d. All of the above e. A and B only 10. Which of the following could be a barrier that prevents successful transformation of single stranded DNA by naturally competent cells? a.
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Exam 3 Answer Key - Exam 3 MIBO 2500 Spring '08 Dr. Walker...

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