W3OutlineLec7 - • Thymosin-Beta4 Sequesters ATP-actin...

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Luis David Gomez 59866865 Bio 192 D103 Cell Biology Week 3 Lecture 7 Outline 1/ 23/08 (No Lec Monday) Cytoskeleton II: Actin Organization G-actin—asymmetric building block polarized molecule as soon as g-actin begins to polymerize ATP (necessary) is hydrolyzed Assembly Actin Treadmilling (+) end—affinity is higher for g-actin, LOW Cc (less actin needed to add here) (-) end—affinity is lower for g-actin Treadmilling requirement: In between Cc of +/- end Regulation 1. Capping protein 2. Stabilizing/destabilizing proteins: Cofilin--destabilizer high affinity for ADP-Actin, removes it to increase treadmiling efficiency increases Cc 3. Sequestering Proteins
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Unformatted text preview: • Thymosin-Beta4 Sequesters ATP-actin Have plenty of ATP-actin BUTbut it would be “tied up” and it would cause filament to shrink Q.Q. How does actin behave in the presence of a – end binding protein and c (cytosolic g-actin-atp less than Cc(+)? C) Because the (-) end binding protein is holding (-) end together then only the (+) end would shrink, Cc (+) is low Dynamics ARP2/3 nucleating protein: induces branching (70 degrees) polymerization of Lysteria: ActA • Necessary—if we remove it will it work? • Sufficient—if we add it will it behaive the same Examples: bacterial invasion cell locomotion...
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