W5OutlineLec12 - Luis David Gomez 59866865 Bio 192 D103...

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Luis David Gomez 59866865 Bio 192 D103 Cell Biology Week 5 Lecture 12 Outline 2/ 4/08 Vesicle Formation Steps of Vesicle Transport 1)Sar-1-GDP is recruited by Sec 12 (GEF) activating the GTPase exposes its lipid anchor 2) CopII coat assembly –curves membrane recruit cargo/integral membrane proteins 3) Once transport occurs, GTP hydrolysis occurs because Sar-1GTP performs its purpose 4) Disassembly of coat Features Transport from/to ER is the same mechanism v-SNARE recognize t-SNARE on Target membrane form a tight complex helps fuse two lipids bilayers after vesicles through GTP hydrolysis after vesicles have traveled down MT highways (dynein/kinesin motors) --cargo is dropped off SNARES helical domain the C-terminus and N-terminus stays the same as topology of protein is determined during co-translational insertion 4 v-SNARE/t-SNARE complex are needed, deletion of 1 leads to poor docking
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