2016 Nov 7 Slides - BIOL14a Nov 7 2016 Vote Tomorrow What...

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BIOL14a Nov 7, 2016 Vote Tomorrow!
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What about transgenic mouse that isn’t knock-out? -We are adding a gene we want to study or use in the animal (e.g., the GFP mice). -Doesn’t necessarily need to be targeted, so may not have to use Embryonic Stem Cells (ESCs)
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Targeted Integration: Select ES cells are added to an embryo to make a Chimeric Mouse - composed of cells of different genotypes
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A chimeric mouse has some transgenic cells, and some normal mouse cells.
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Chimeric mice are bred to produce mice that have only transgenic cells: fully transgenic mice
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Chimeric mouse and her fully- transgenic offspring
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Non-targeted integration: -We are adding a gene we want to study or use in the animal (e.g., the GFP mice). -If non-targeted, we can modify a zygote directly instead of using ESCs.
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DNA is injected into pronucleus of recently fertilized egg
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Surrogate mother mouse and her fully- transgenic offspring
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DNA is injected into pronucleus of recently fertilized egg Problems: -no control over where transgenic DNA is integrated into genome!
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