2016 BIOL14 Nov 2 Slides (1)

2016 BIOL14 Nov 2 Slides (1) - Nov 2 2016 Gene cloning and...

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Nov 2, 2016 Gene cloning and Transgenic Organisms
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Gene Cloning Example Diabetes and insulin production
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An example of cloning a specific gene: Goal: make bacteria that will make human insulin for us! Lots of human insulin!
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Goal: make bacteria that will make human insulin for us! Lots of human insulin!
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Cloned Insulin gene Recombinant Plasmid Vector Bacterial genome
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Do your best to draw a diagram of the recombinant plasmid vector you need for this, including 4 key features!
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Does our vector have everything it needs for this goal? Lots of human insulin!
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Basic Components/Tools for Cloning Vector Source of insert (donor) DNA: Genomic DNA (gDNA) Human gDNA includes introns cDNA the DNA copy of the mRNA No introns Other
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The sequence of insulin cDNA: 1 agccctccag gacaggctgc atcagaagag gccatcaagc agatcactgt ccttctgcca 61 tggccctgtg gatgcgcctc ctgcccctgc tggcgctgct ggccctctgg ggacctgacc 121 cagccgcagc ctttgtgaac caacacctgt gcggctcaca cctggtggaa gctctctacc
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