final exam key - Name _KEY_ BIOLOGY 325H Genetics Spring...

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Name _______KEY ___________ 1 BIOLOGY 325H Genetics Spring 2008 Final Examination (160 points) Before you do anything else , please: check to make certain that your copy of the exam is complete, i.e. 9 pages. print your name legibly in the space provided at the top of each page. 1. [15 pts] You’re working with a plant that is not a model organism, Deloviliana priklianus , so its genome has not been sequenced. Nonetheless you think it will be ideal for learning about the genetics of spines. You’ve already created a genetic map of D. priklianus so you have a lot of genetic markers, approximately 1 every 10 centiMorgans. Now you want to use a set of RILs to determine the parts of the chromosomes where the genes for spines are located. A . What does RIL stand for and how might you produce your own set of them for your species? Assume your species has hermaphroditic flowers and is self-compatible. Also assume you have four potential parents from which you can pick only two. Explain why you pick the parents that you do. Parent 1: long wide spines Parent 2: long thin spines Parent 3: short wide spines Parent 4: medium thin spines A RIL is a recombinant inbred line. Since the goal in producing a set of RILs is to have a set of inbred lines that allow you to determine the regions on one or more chromosomes where the genes are that produce a quantitative trait (in this case different types of spines), it is best if the parents used to make the RILs are as different as possible in the trait(s) of interest. In this case, you would cross parents 2 and 3 since their spine types are the most different (long-thin and short-wide). To make your RILs you would cross parents 2 and 3 and then take many of the F 1 seeds (up to several hundred) and use single-seed-descent lines for each seed where you self each line for eight or nine generations. B . You’ve produced your RILs, what sort of patterns will you look for to determine the chromosomal locations of genes responsible for different types of spines? Using the genetic maps that you have for the parents and the RILs that you made, you should look for correlations between particular markers in the RILs and the different spine characteristics (long vs. short and wide vs. thin). Markers that are highly correlated with particular forms of the trait indicate chromosomal locations where a gene or genes for the trait are located. [Given the density of the genetic maps (1 marker each 10 cM), you should be able to narrow down the location of the genes to a few 10s of cM.]
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Name _______KEY ___________ 2 2. [20 pts] D. priklianus grows in a forested environment where global climate change is expected to increase the temperature and decrease the average amount of precipitation. The decreased precipitation will reduce other food resources for its main herbivore, rabbits,
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This note was uploaded on 05/14/2008 for the course BIO 325H taught by Professor Staff during the Spring '08 term at University of Texas at Austin.

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final exam key - Name _KEY_ BIOLOGY 325H Genetics Spring...

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