plasmid transformation

plasmid transformation - Joshua Sipe Biology104 SectionM01...

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Joshua Sipe Biology104 SectionM01 May 5, 2008 Title : Plasmid Transformation of E. coli Abstract: The objective of this lab was to create competent bacterial cells able to pick up plasmids and express the genes from these plasmids, changing their phenotype. Using calcium chloride to enable the E. coli to uptake DNA; ampicillin and X-gal were added to the E. coli so that the E. coli would express different genes from each treatment. Two samples were created, one containing E. coli and the other containing ampicillin genes and X-gal genes. The genes containing only E. coli were added to the agar plates on the – side while the sample containing ampicillin and X-gal were added to the + side. The different samples were allowed to incubate for a period of a few days allowing different colonies of E. coli to grow. On the control plate E. coli bacteria was able to grow on both sides of the plate. On the Luria broth plate containing ampicillin the E. coli was able to grow on the positive side only. On the Luria broth
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This note was uploaded on 04/16/2009 for the course BIO 104 taught by Professor Dr.smith during the Spring '09 term at SUNY Adirondack.

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plasmid transformation - Joshua Sipe Biology104 SectionM01...

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