Chapter 8 Outline

Chapter 8 Outline - Chapter 8 Gene Transfer Genetic...

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Chapter 8 Gene Transfer, Genetic Engineering, and Genomics Introduction 8.1 Genetic Recombination in Prokaryotes Genetic Information in Prokaryotes Can Be Transferred Vertically and Horizontally Vertical gene transfer (VGT) is the transfer of genetic material from parent cell to daughter cell (through binary fission) Horizontal gene transfer (HGT) is involved the transfer of DNA from a donor cell to a recipient cell Transformation Is the Uptake and Expression of DNA in a Recipient Cell By integration of a new DNA fragment, the recipient has gained some ability it previously lacked Transformation was first described by Frederick Griffith in 1928 Competence refers to the ability of a recipient cell to take up DNA from the environment Conjugation Involved Cell-to-Cell Contact for Horizontal Gene Transfer In conjugation, a donor cell transfers DNA directly to the recipient It was first observed in 1946 by Joshua Lederberg and Edward Tatum The donor cell forms a conjugation pilus to make contact with the recipient The F factor DNA in the donor replicates by the rolling-circle mechanism Conjugation also Can Transfer Chromosomal DNA High frequency of replication (Hfr) strains of prokaryotes can donate chromosomal genes rather than just the F plasmid The F factor attaches to the chromosome using an insertion sequence
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Chapter 8 Outline - Chapter 8 Gene Transfer Genetic...

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