2203 Lab Report 1

2203 Lab Report 1 - Melinda Salomon Genetics 2203 Section...

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Melinda Salomon Genetics 2203 Section 19 Lab Report 1 Drosophila Melanogaster Cross #1 Segregation/Independent Assortment OJECTIVE : To determine if the traits dumpy and sepia follow Mendel's Laws of Inheritance. MATERIALS AND METHODS : Two sepia virgin Drosophila melanogaster females and five dumpy Drosophila melanogaster males were placed in a vial containing agar. The alternative cross performed in the lab section was two dump virgin Drosophila melanogaster females and five sepia Drosophila melanogaster males. CO2 was used to anesthetize the flies. After one week, P1 Drosophila melanogaster had laid eggs and F1 pupas were visible. P1 flies were removed from vial. One week later, F1 Drosophila melanogaster had developed and were analyzed and counted. A new vial with agar was setup containing two Drosophila melanogaster F1 females and five Drosophila melanogaster F1 males. After one week, pupas were visible and F1 parentals were removed. One week following, the developed F2 Drosophila melanogaster were analyzed and counted according to both sex and phenotype. A second vial was setup with agar containing two Drosophila melanogaster F1 dumpy sepia females and five Drosophila melanogaster dumpy sepia males. The alternative crosses performed in this section were two Drosophila melanogaster dumpy sepia females and five Drosophila melanogaster F1 dumpy sepia males, two Drosophila melanogaster dumpy sepia females and five Drosophila melanogaster F1 sepia dumpy males, two Drosophila melanogaster F1 dumpy sepia females and five Drosophila melanogaster sepia dumpy males. After one week, pupas were visible and parentals were removed. One week following, the developed F2 Drosophila melanogaster were analyzed and counted according to both sex and phenotype. This cross served as a test cross. RESULTS
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This note was uploaded on 05/29/2008 for the course BIOLOGY 2203 taught by Professor Anthwal during the Spring '08 term at Temple.

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2203 Lab Report 1 - Melinda Salomon Genetics 2203 Section...

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