2203 Lab Report 2

2203 Lab Report 2 - Melinda Salomon Genetics 2203 Section 19 Lab Report 2 Drosophila Melanogaster Cross#2 Sex Linkage and Mapping OJECTIVE To

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Genetics 2203 Section 19 Lab Report 2 Drosophila Melanogaster Cross #2 Sex Linkage and Mapping OJECTIVE : To determine if the traits yellow body color, crossveinless wings, miniature wings, white eyes, and forked bristles are sex-linked traits in Drosophila melanogaster and to determine the mapping sequence. MATERIALS AND METHODS : Three crosses were performed. Cross A: Two yellow, crossveinless virgin females and five dumpy males. Cross B: Two yellow virgin females and five miniature winged, forked bristled males. Cross C: Two miniature winged virgin females and five yellow, crossveinless males. The flies for each cross were placed in three respective vials containing agar. CO2 was used to anesthetize the flies. After one week, P1 flies had laid eggs and F1 pupas were visible. P1 flies were removed from each vial. One week later, F1 flies had developed for each cross and were analyzed and counted. Three new vials with agar were setup containing two F1 females and five F1 males respectively. After one week, pupas were visible and F1 parentals were removed. One week following, the developed F2 males of each cross were analyzed and counted according to phenotype. RESULTS : Cross A parental: y/y cv/cv w+/w_ X y+/y_ cv+/cv_ w/w Parental Punnett Square: y cv w+ y cv w- *(- denotes can be either + (wild) or not) y+ cv+ w y/y+_ cv/cv+ w+/w y/y+_ cv/cv+ w-/w y- cv- w y/y-_ cv/cv- w+/w y/y-_ cv/cv- w-/w y+ cv- w y/y+_ cv/cv- w+/w y/y+_ cv/cv- w-/w y- cv+ w y/y- cv/cv+ w+/w y/y- cv/cv+ w-/w Cross B parental: y/y m+/m_ fl+/fl_ X y+/y_ m/m fl/fl Parental Punnett Square: y+ m fl_ y- m fl *(- denotes can be either + (wild) or not) y+ m+ fl+ y+/y+_ m/m+ fl+/fl y-/y+_ m/m+ fl/fl+ y- m- fl+ y+/y-_ m/m- fl+/fl y-/y-_ m/m- fl/fl+ y+ m- fl+ y+/y+_ m/m- fl+/fl y-/y+_ m/m- fl/fl+ y- m+ fl+ y+/y- m/m+ fl+/fl y-/y- m/m+ fl/fl+ y+ m+ fl- y+/y+_ m/m+ fl/fl- y-/y+_ m/m+ fl/fl- y- m- fl- y+/y-_ m/m- fl/fl- y-/y-_ m/m- fl/fl- y+ m-
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This note was uploaded on 05/29/2008 for the course BIOLOGY 2203 taught by Professor Anthwal during the Spring '08 term at Temple.

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2203 Lab Report 2 - Melinda Salomon Genetics 2203 Section 19 Lab Report 2 Drosophila Melanogaster Cross#2 Sex Linkage and Mapping OJECTIVE To

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