biolab1 - Anzalone 1 Christopher M. Anzalone Biological...

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Anzalone 1 Christopher M. Anzalone Biological Inquiry II Section 02 Dr. Brady Porter March 6, 2008 The Determination of Relationships Among Land Plants, Green Algae, Red Algae, and Brown Algae Based on Related Photosynthetic Pigments. Introduction : Over the years, biologists have focused on classifying organisms based on their morphological similarities. More recently, biologists have changed their approach of classification and have begun classifying organisms based on molecular phylogeny. This method characterizes organisms based on synapomorphies, which are the organism’s shared derived characteristics. In order to conduct this experiment, the process of thin layer chromatography was utilized. This process arranges an organism’s organic pigments based on size, and with the aid of a spectrophotometer, we can determine which wavelengths are absorbed by the organism’s pigment (Freeman 206-207). This experiment focuses on the presence of different photosynthetic pigments in the individual organism studied. Emphasis is placed on similarities in pigments among the various species of the different taxa examined. Due to the presence of the individual pigments, creates a basis for evolutionary relationships to be proposed. The null hypothesis for this experiment states that there will be no relationship between the pigments observed. The alternative hypothesis states that the observed pigments will correspond to an evolutionary relationship. More specifically, land plants will have
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Anzalone 2 increased pigmentation as compared to the other species examined. The basis for this hypothesis results from the belief that land plants must compete more readily for light sources due to their closer proximity and thus must be able to absorb wider ranges of light for photosynthesis. Materials and Methods : To begin the experiment, the pigments that were to be analyzed were obtained. These pigments included chlorophyll and carotenoids pigments from land plants, green algae, red algae, and brown algae. The extracts are placed at the bottom of filter paper and placed into a solvent solution. The solvent interacts with the
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biolab1 - Anzalone 1 Christopher M. Anzalone Biological...

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