mt1answerkey

mt1answerkey - 1) The structural integrity of...

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Unformatted text preview: 1) The structural integrity of supramolecular and biomolecular complexes (assemblies) of multiple components are bonded to each other by all of the following forces EXCEPT : (circle one choice only). (5 pts) Covalent bonds l . Van der Waals forces c. Hydrogen bonds (1. Hydrophobic interactions e Ionic interactions 2) Which of the following weak acids would make the best buffer at pH = 7.0? (circle one choice only). (5 pts) Formic acid (K, = 1.78 x 10") b) , Imidazole (Ka = 1.02 x 10-7) c Bicarbonate (Ka = 6.3 x 10'“) d) Phosphoric Acid, pK2 (K“ = 6.31 x 10"”) Briefly explain your answer: (5 pts) (“4‘ Qooycwt ’54 a~~ 5;; ‘7“; i J K 3) pH = pKa when: (5 pts) 3) 10g ([A-]/ [HAD = 1 b [A-]>>[HA] @iA-i = [HA1 [A-]/[HA] = 0 e) 10g(lHAl/[A-])= 1 4) All are distinctive properties of living systems EXCEPT: (5 pts) iving organisms are relatively simple I Biological structures play a role in the organism’s existence c) The living state is characterized by the flow of energy through the organism d) Living systems are highly organized e) Living organisms are actively engaged in energy transformation 5) Write all structures for the ionic dissociation of Glutamic Acid as you progress from low to high pH. Include ALL species and identify the charge state for each species. Write the single and three letter code for this amino acid. (15 pts) 33’». .4,»- ‘4 g; H N m cm 6 c2 c9 s4 W3.» Ni if; w c o 0 V? . r I w c.7145 , é .. C H g r; (é ,, w. +3 g ,1 h @343 .a O z a v 6) List the five subtypes of secondary (2°) protein structure. (15 pts) n__ we; m\ k j 3 g - 4; ‘ , ,1 '2 f ,1 ’1”: i_ EM" i7/ Lew!" " CM a. ,,,, ,. 7) Draw the titration curve for Arginine and ALL of its structures as you progress from a point where M2 base has been added to high base concentration. Assume that you are titrating with sufficient base to reach pH 14. Indicate the charges on this amino acid at the start point and at each of the pK values and calculate the isoelectric point. (Show all your work). (25 pts) % 8) What is the pH of a 30 “M solution of HCl? (10 pts) EM» if} H ‘7' m: 3' O )</ a)“ Q ~- V g I, “"\ v L7. 5; a *0 w“ 9 a) What is the pH of a 100 ml solution of 0.5 M benzoie acid? )(a or benzoic acid = 6.31 x 10‘5 (whole problem IS worth 25 pts). P H :7 (P 15K “k ‘ W {WA “1 ”@m M “w i J; H A «w “it it / flgg W X r :4: 9 x 5:?ééw‘l f/ .z‘ t W a. a: :5? <3 «it; s “xx 1 v 4 Z I <1 I ; k‘é ’ , E 1 “x b) What is the new pH of the solution from problem 9(3) above) if you add 100 ml of 0.2 M NaOH and bring the total volume up to 1 L? What is the pOH? rm»; ‘ “L g ’m' M‘s la 3%,” MA], C jiGN‘: r: M um) ~- V "L y. t W... MWz‘wwwexchflMJW—ww {L 1‘ l a; i X :3 N: xv hi ,z". g/MW N“? lg: r- ., If [2’ O 4 :5" a; *“ ~ 3 2553 ' :r LOH1“ikO’V,kMLfo ‘ &%§%WJE ;” t i V bf:- <v , I W. a”; f ‘21; t 6" " F‘ f m: 1 in} \ to“ 2 V ?“**52 POH“’V *“s‘ g: sky“ a ‘3 ; Wflfi $?_ j g {ah “ is, W; w r} ' I, ’ 5 10) You have been given an octapeptide (Pep8) whose composition and sequence you need to determine. You are given the following information below for several steps along the way. NOTE: Some information will be more than you need (duplicate) to solve the final sequence. 1) Total acid hydrolysis and ion exchange chromatography indicate that you have the following amino acid composition: arg, glu, ile, lys, met, phe, ser, val 2) Trypsin digest produces 1 tryptic tripeptide, 1 tryptic tetrapeptide and a single amino acid. What information do you have at this point about the sequence of Pep8? (3 pts) _. ., {in M( CW3?» \ . -"‘"" » "‘ ‘5'“;WAJ-fi muff is“) {:5 33‘" "i .423 G Mung—Anna— 3) Further AA analysis of the tryptic tripeptide and tetrapeptide (from step 2 above) indicates the composition of these two peptides to be ile, lys, val for one, and arg, glu, met, phe for the other one. What new information do you have at this point about the sequence of Pep8? (3 pts) .964.) wt W431 ; iii‘e—b—‘iaut 9L 4) Carboxy peptidase treatment of Pep8 produces the amino acid ser. What new information do you have at this point about the sequence of Pep8 ? (3 pts) K k \ ‘> SW 4— ""1 5) FDNB (Sanger’s reagent) reaction of Pep8 produces the amino acid ile. What new information do you have at this point about the sequence of Pep8 ? (3 pts) . u-L/MM :3 w ‘ 4 » ,i «f» if “fL’YYfi'L‘x-cA I I t w 4 " KEN a j, » J W ‘E y\\ H 6 «a. ‘ f? , ,5? a}; M‘VAM‘QWK; ,4” fi ; m w) M i i , , KT 6) Cyanogen bromide of Pep8 produces two tetrapeptides What new information do you have at this point about the sequence of Pep8 ? (3 pts) . I f C n» deaf: VA‘J’W ’ J l/ i r‘ i a». 1 W A“ (firing was; “graffiw‘ J m; a w» p j 4w M flaw a a 7) Chymotrypsin reaction of Pep8 produces 1 pentapeptide and 1 tripeptide. Mass spectral analysis tells you that the C-terminal amino acid of the pentapeptide is Phe; What new information do you have at this point about the sequence of Pep8? (3 pts) “my Mi.) 2: u M ,M w» M Q/‘NJ/ N 1 w “ {fie-m 4 "-1 3‘) u r Ra aw» 8) Staphylococcal protease reaction of Pep8 produces a hexapeptide which now leaves a remaining dipeptide 5, 2 x, , txg What new information do you have at this point about the sequence of Pep8? (3 pts) What is the sequence of the original Pep8 octapeptide? (4 pts) w” M {Mfg Mm ‘ «was: m 45‘ ,, 6M W «is! kw L:- 11) Give the single letter code for the following peptide. (10 pts) Cys — Ala — Arg — Asp — Ile — Asn - Ala — Leu — Ser — Trp — Ile —- Asn !! C». 9: {21-.. b N A» #3} mm); A} (H I Is that a good thing? SW V F” vu\vxg§£f ’ J ...
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This note was uploaded on 05/24/2008 for the course BIS 102 taught by Professor Hilt during the Fall '08 term at UC Davis.

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mt1answerkey - 1) The structural integrity of...

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