Liam Bio Lab 2 - Permeability of the Red Blood Cell By Liam...

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Permeability of the Red Blood Cell By Liam P. Charron 8199291 BIO1140 Section B4 Demonstrators: Jonathan Tea and Demissew Sertse Desta February 25 th 2016 Department of Biology University of Ottawa
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Table 1: Hemolysis times of red blood cells of horses in 5 different solutions (Set B), based on 3 trials for each solution. Hemolysis times based on the elapsed time for 75% of cells to hemolyze. Elapsed Hemolysis Time 1 Solution Trial 1 (s) Trial 2 (s) Trial 3 (s) Mean HT (s) Standard Error Distilled Water 2 2 2 2 0 Ethanol [0.3M] 2 2 2 2 0.00 Triton X-100 [2%] 2 2 2 2 0.00 Thiourea [0.3M] 51 54 51 52 1.00 Dextrose [0.3M] 1200 1200 1200 1200 0.00 1 Solutions with hemolysis times of > 2s hemolyzed instantaneously and were inserted into the table as 2s, while solutions with hemolysis times of < 1200s did not hemolyze within 20 minutes, and likewise their hemolysis times were inserted into the table as 1200s so that the Mean/Standard Error calculations could be efficiently performed.
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Interpretation: 1. Factors that affect solute diffusion include: molecule size, with larger molecules diffusion slower through the channels in the membranes. Ions pass through nearly
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